The Amplification and Activity Analysis of the Small Rubber Particle Protein (SRPP) Promoter of Eucommia ulmoides (EuSRPP) Revealed That Its Activity Was Regulated by MeJA, GA3, and Drought Pathways

As an important temperate gum source plant, Eucommia ulmoides is widely distributed in China, but the low yield of considerably affects its application as a natural rubber practical production. The small particle protein (SRPP) gene influential participant biosynthesis process, and expression content. In this study, promoter activity SRPP (EuSRPP) was analyzed by molecular biology bioinformatics. order to understand regulation mechanism EuSRPP genes at transcriptional level, we first obtained sequences EuSRPP1, 3, 4, 5, 6, 7 via genome walking PCR amplification experiments. Then, T3 generation transgenic homozygous line genetic transformation Arabidopsis thaliana mediated Agrobacterium. six promoters were expressed plants stably leaves, pollinated flowers, mature pods. plant grows develops, root barely expressed. addition, after treated with methyl jasmonate (1 mmol/L MeJA), gibberellin GA3), drought (20% PEG6000), increased then decreased. difference, however, that 4 reach their strongest GUS 3 h treatment, while EuSRPP5, 6 treatment. Based on all experimental results, for time, it has been shown loci relatively consistent. Second, different under MeJA, GA3, suggesting regulated endogenous hormones pathways.